Step 4: stamping the enzyme into the cellulose acetate gel
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Place alignment base near the sample plate with the words "helena laboratories" facing you
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place a folded sheet of blotting paper adjacent to alignment base
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remove gel from buffer and place it between the folded paper dull side up
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gently press to remove excess buffer
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place a drop of buffer on the center of alignment base
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remove applicator from sample plate
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press applicator down 2 or 3 times
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with "super Z" facing you, place the two legs on the plate so that the numbers on the applicator correspond to wells on sample plate
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alignment base so that the edge of the gel labeled with (-) matches up with the line on the alignment base labelled "cathode application"
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with dull surface up, place gel on the alignment base.
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place the applicator into the alignment base again so that "super Z" is facing you
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press pins of applicator down onto the gel for 5 seconds (do no press too hard!)
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all eight pins should have transferred their samples, leaving 8 green bands
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take the gel to the electrophoresis chamber
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