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Obtain six petri dishes containing synthetic "dropout" medium "SD-trp" label one petri dish as no fabric and the rest with either cotton, polyester, linen, nylon, or rayon Place the six petri plates on the bench right side up obtain an original yeast suspension with cells that bears the allele trp1-289 pipette 100 micro liters of the suspension onto each petri dish replace cover of each dish wait a few seconds for the spreader to cool, then carefully spread the suspension over the entire plate remove the glass spreader from the alcohol and pass it through the flame place a glass spreader in a beaker containing 95% alcohol and light a flame replace the cover of each dish after pipetting make a foil cap for the UV irradiation lamp and turn on the short wave UV lamp and cover with cap cover each plate with the fabric labelled on the dish. The no fabric dish should only have saran wrap doing two at a time: place the dishes under the lamp and remove the cap allow the petri dishes to sit under the lamp for the number of seconds found in the previous lab that induces mutations. replace cover for each dish and tape all 6 together upside down. allow plates to be induced at 30 degrees for 2 days & then placed in a refrigerator