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Set wavelength of spectrophotometer to 605 nm
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put 15 ml of CMS into 30 ml cold beaker with a plastic beaker as an ice bucket on the bottom
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Obtain one tube of DCIP and 15 ml of water in flask
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obtain 7 test tubes. First one labelled B for blank and the red #1-6
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Add 0.5 ml of water and 2.5 CMS to blank tube
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place tube #1 in foam pad with the lights off
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Cover with parafilm, mix well and record absorbance and time.
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Tube #1- 0.5 ml DCIP and 2.5 ml of water
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Use blank to zero spectrophotometer
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Cover blank with parafilm and mix well
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turn on light and expose test tube #! for one minute
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Leaving the light on and covering the tube, record test tube #1's absorbance
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Repeat last two steps until you have 5 absorbances
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Test tube #2- add 0.5 ml DCIP, and 2.5ml of CMS
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Repeat steps 11-12 with test tube #2 five times and record all absorbances
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Mix tube well then remove foil jacket and cap
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Cover test tube #3 with parafilm along with the cap and aluminum foil jacket.
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Test tube #3- add 0.5 ml of DCIP, and 2.5 ml of CMS
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prepare a foil cap for test tube #3
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Wrap test tube #3 with aluminum foil
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quickly measure the absorbance of the tube at 605 nm
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Put the jacket and cap back on test tube #3 and place it in the light box and record the time
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turn on the light and record the absorbance every 1 minute 5 times while reducing the amount of light each time by removing the cap and jacket
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Cover the light box with purple cellophane and turn on the light
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Test tube #4- add o.5 ml DCIP and 2.5 ml of CMS and mix well
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For test tube #6 repeat steps 24-28 with red cellophane
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For test tube #5 repeat steps 24-28 with green cellophane
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Record the absorbance and repeat step 27 until 5 readings are made.
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Place test tube #4 under in the light box with the purple cellophane for 1 minute
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Take the absorbance of Test tube #4
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For test tube #7 repeat steps 24-28 with another colored cellophane
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open Microsoft Excel and enter time(x-axis) and absorbance at 605 nm (y-axis) on adjoining columns
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highlight the date and select chart wizard
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Click XY scatter chart type
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Highlight the columns button
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Light driving Photosynthesis
