Determine one of the four species
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Positive gram stain
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YES
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Gram negative stain
week 12
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YES
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Negative gram stain
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Negative stain
week 12
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Fluid Thio-
gylcollate
Medium
Week 12
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Fluid Thio-
gylcollate
Medium
Week 12
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Negative stain week 12
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Blood agar plate
week 12
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Capsule stain
week 12
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If positive for capsule
species is
Enterbactor
aerogenes
keep testing for character-istics
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Capsule stain
week 12
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MacConkey
agar
week 12
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SIM
week 12
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Mannitol/
Lactose
via phenol red broth
week 13
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If negative for capsule,
species could be Salmonella
choleraesuis or
Proteus
morabilis
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KIA
week 13
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Mannitol/
Lactose
via phenol red broth
week 13
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KIA
week 13
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Catalase
week 13
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Catalase
week 13
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Methyl red-
Voges-
Proskauer
week 13
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Bile esculin
week 13
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CAMP
SXT
Bacitracin
week 13
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Urease
week 13
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Nitrate
week 13
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Optochin
week 13
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Strepto-
coccus
pyogenes
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If species doesn't swarm it is Salmonella
cholerasuis
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If species swarms, it is Proteus
morabilis
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Swarming test
week 13
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Unknown 2
